The restaurant posted photos of the employees delivering food to people waiting in line to donate blood to the victims. The post said, "We love our city and love the people in our community. #prayfororlando."
Many of the 49 killed and 53 wounded were members or allies of the LGBT community. The act of kindness follows several years of tension between that community and the restaurant chain.
Chick-fil-A declined to comment, but a spokesperson for its Florida stores told Fox News, "We are just honored to be one of many organizations that are helping our friends and neighbors in the Orlando community during this difficult time."